Quorum Sensing

limit the amount of homoserine lactone that you may use in the

assay.

6. The kinetic assay uses the change in absorbance at 577 nm of
   m-cresol purple (Fig.3).


7. You may use graphing programs such as Prism or Enzfitter to
   determine the kinetic parameters.

Fig. 2Chemical structures of different AHLs

Fig. 3Schematic representation of the reactions carried out by the GKL lactonase. The assay used to quantify
GKL activity is based on the GKL-dependent conversion ofm-cresol purple to the yellow form (maximum
absorbance at 577 nm)

322 Maybelle Kho Go et al.