Contents
Foreword.................................................................... v
Preface. .................................................................... vii
Contributors................................................................. xiii
PARTIDETECTION ANDQUANTIFICATION OFQUORUM
SENSINGSIGNALMOLECULES
1 Use of Whole-Cell Bioassays for Screening Quorum Signaling,
Quorum Interference, and Biofilm Dispersion.............................. 3
Starla G. Thornhill and Robert J.C. McLean
2 Detection of 2-Alkyl-4-Quinolones Using Biosensors....................... 25
Matthew P. Fletcher, Stephen P. Diggle, Miguel Ca ́mara,
and Paul Williams
3 “Hot Stuff”: The Many Uses of a Radiolabel Assay
in Detecting Acyl-Homoserine Lactone Quorum-Sensing Signals............. 35
Amy L. Schaefer, Caroline S. Harwood, and E. Peter Greenberg
4 Liquid Chromatography/Mass Spectrometry (LC/MS)
for the Detection and Quantification ofN-Acyl-L-Homoserine
Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs). ................ 49
Franc ̧ois Le ́pine, Sylvain Milot, Marie-Christine Groleau,
and Eric De ́ziel
5 Detection of the Bacterial Quorum-Sensing Signaling
MoleculesN-Acyl-Homoserine Lactones (HSL)
andN-Acyl-Homoserine (HS) with an Enzyme-Linked
Immunosorbent Assay (ELISA) and via Ultrahigh-Performance
Liquid Chromatography Coupled to Mass
Spectrometry (UHPLC-MS)............................................. 61
Michael Rothballer, Jenny Uhl, Josie Kunze, Philippe Schmitt-Kopplin,
and Anton Hartmann
6 Biosensors for the Detection and Quantification of AI-2 Class
Quorum-Sensing Compounds............................................ 73
Sathish Rajamani and Richard Sayre
7 Detection ofAgr-TypeAutoinducing Peptides Produced
byStaphylococcus aureus.................................................. 89
Ewan J. Murray and Paul Williams
8 Ultra-Performance Liquid Chromatography/Mass Spectrometry
for the Detection and Quantification of Diffusible Signal
Factor (DSF) Family Quorum-Sensing Signals.............................. 97
Lian Zhou, Shuang Sun, Wei Zhang, and Ya-Wen Heix