9 Rapid Electrochemical Detection ofPseudomonas aeruginosa
Signaling Molecules by Boron-Doped Diamond Electrode................... 107
Alyah Buzid, John H.T. Luong, F. Jerry Reen, Fergal O’Gara,
Jeremy D. Glennon, and Gerard P. McGlacken10 Detection and Quantification of Butyrolactones fromStreptomyces............ 117
Marc Biarnes-Carrera, Rainer Breitling, and Eriko Takano
PARTII METHODS FOR THESTUDYING OFQUORUMSENSING AT THE
MOLECULAR,PHYSIOLOGICAL,ANDPOPULATIONLEVEL
11 Fluorescence Quenching Studies ofγ-Butyrolactone-Binding
Protein (CprB) fromStreptomyces coelicolorA3(2)........................... 131
Jessy Mariam and Ruchi Anand
12 Methods to Study Solo/Orphan Quorum-Sensing Receptors ................ 145
Vittorio Venturi, Sujatha Subramoni, Anice Sabag-Daigle,
and Brian M.M. Ahmer
13 Enzymatic Assays to Investigate Acyl-Homoserine Lactone
Autoinducer Synthases.................................................. 161
Daniel Shin and Rajesh Nagarajan
14 Global Expression Analysis of Quorum Sensing-Controlled
Genes by RNAseq. ..................................................... 177
Charlotte D. Majerczyk
15 Identification of AHL- and BDSF-Controlled Proteins
inBurkholderia cenocepaciaby Proteomics.. ............................... 193
Yilei Liu, Gabriella Pessi, Katharina Riedel, and Leo Eberl
16 ImagingN-Acyl Homoserine Lactone Quorum Sensing In Vivo ............. 203
Louise Dahl Hultqvist, Maria Alhede, Tim Holm Jakobsen,
Michael Givskov, and Thomas Bjarnsholt
17 AssessingPseudomonas aeruginosaAutoinducer Effects
on Mammalian Epithelial Cells........................................... 213
Jake Everett, Rebecca Gabrilska, Kendra P. Rumbaugh,
and Elena Vikstro ̈m
18 Animal Models forPseudomonas aeruginosaQuorum Sensing Studies ......... 227
Damien Maura, Arunava Bandyopadhaya, and Laurence G. Rahme
19 Methods to Study Quorum Sensing-Dependent Virulence
and Movement of Phytopathogens In Planta ............................... 243
Shulamit Manulis-Sasson and Laura Chalupowicz
20 Differential Equations Models to Study Quorum Sensing.................... 253
Judith Pe ́rez-Vela ́zquez and Burkhard A. Hense
PARTIII IDENTIFICATION ANDCHARACTERIZATION OFANTI-QUORUM
SENSINGAGENTS
21 Qualitative and Quantitative Determination of Quorum Sensing
Inhibition In Vitro. ..................................................... 275
Tim Holm Jakobsen, Maria Alhede, Louise Dahl Hultqvist,
Thomas Bjarnsholt, and Michael Givskov
x Contents