RNA Detection

Press the injection button on the joystick to inject the target RNA solution into the cell. The needle will return to the searc ...

background fluorescence even when closed, due to imperfect quenching of the fluorophore by the quencher. Thus, when delivered in ...

It is recommended to allow microemulsion bubbles to settle on the imaging surface (cover glass) for a few minutes before imagin ...

and primary BJ cells. Additionally, we also found that for a different set of MBs with unique stem and loop lengths and sequence ...

target RNA. Nucleic Acids Res 36(12):e69. doi:10.1093/nar/gkn331 Wu Y, Yang CJ, Moroz LL, Tan W (2008) Nucleic acid beacons for ...

Chapter 17 Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH Dan Ohtan Wang Abstract Multiplex RNA det ...

spatiotemporal resolution for understanding and manipulating nuclei in both physiological and pathological states. Newly develop ...

Fig. 1Designing effective sequences of ECHO probes and in vitro characterization. (A) Transcript-specific ECHO probes (black lin ...

2.2 In Vitro Measurement of Fluorescence Activation Kinetics Stopped-flow spectrafluorometer (e.g., Applied Photophysics Model ...

3% agarose/ACSF. Collagen solution (e.g., Nitta Gelatin Cellmatrix). 2.5 Image and Statistical Analysis Image J (NIH). Imaris ...

after mixing.Kis then plotted against the concentrations of target oligonucleotides (Fig.1c). To calculate effective affinityKD ...

Clean the head–neck region of the anesthetized mice with a piece of Kimwipes wet with 70% ethanol then sterile 1PBS immediatel ...

Fig. 2In vivo electroporation procedure to deliver ECHO probes into living mouse brains. (A) Our experimental setup to conduct i ...

(Optional) Add drug solutions directly into the soaking ACSF when necessary. Alternatively, the brains can be cut along the sag ...

Fig. 3Target recognition specificity validated by fluorescence patterns and colocalization with protein markers. (A) Preparation ...

concentration at nuclear speckles cannot be recapitulated with a Cy5-dye labeled oligonucleotide DNA probe, indicating that both ...

4 Notes The interaction of TO also adds thermal stability to the probe: DNA/RNA duplex, indicated by a 7–9C increase inTmfor 1 ...

Lee YB, Chen HJ, Peres JN, Gomez-Deza J, Attig J, Stalekar M, Troakes C, Nishimura AL, Scotter EL, Vance C, Adachi Y, Sardone V ...

Ohmachi M, Fujiwara Y, Muramatsu S, Yamada K, Iwata O, Suzuki K, Wang DO (2016) A modified single-cell electroporation method f ...

Chapter 18 In Vivo Visualization and Function Probing of Transport mRNPs Using Injected FIT Probes Jasmine Chamiolo, Imre Gaspar ...