Fig. 5(a) Imaging SRB-2 aptamer in liveE. coliwith SR-DN dye. Bacteria were transformed with either pET-
SRB2 or pET-tRNA plasmid. Transcription was induced with IPTG and bacteria were incubated with 1μMof
SR-DN (red) for 10 min and imaged at 37C. (b) Imaging DNB aptamer in liveE. coliwith various
fluorophore–dinitroaniline conjugates. Bacteria were transformed with either pET-DNB or pET-tRNA plasmid.
Transcription was induced with IPTG. Bacteria were incubated with 1μM of RG-DN (green), TMR-DN (yellow),
SR-DN (red) or TR-DN (magenta) for 10 min and imaged at 37C. Scale bar, 3μm
Visualizing RNA with Fluorogenic Aptamers in vivo 297