Endophytes Crop Productivity and Protection Volume 2 (Sustainable Development and Biodiversity)

(Tina Meador) #1

BH72 has been studied for nitrogenfixation abilities at genomic level and complete


genome was matched with related soil- and plant-associated bacteria (Hurek and


Reinhold-Hurek 2003 ; Krause et al. 2006 ). Many plant-associated bacteria and


pathogens contain N-acyl homoserine lactone-(AHL) for quorum-sensing system


(Preston et al. 2001 ; Buttner and Bonas 2006 ). Nevertheless, (Krause et al. 2006 )


another factor for plant microbe interaction has been identified encoded by BH72


genome. It includes Tpe I and II protein secretion system with Type IV surface


polysaccharides on pili, andflagella so as to produce chemotaxis proteins and ferric


siderophore uptake systems. The valuable biological insight was provided by BH72


genome. In this perspective, it is clear that as much genome sequences of endophyte


will be available, much exploration of the mechanisms involved in successful


endophyte colonization.


7.5 Post Genomic View of Bacterial EndophyteS


Metagenomics analysis of endophytic bacteria associated with rice plant confirmed


few traits that also exist in endophytes. Therefore, these traits evaluated through


genomic analysis become potentially important for their interactions with plants.
These include entire set of highly specialized bacterial secretion system, except type


III due to its non-conserved nature among endophytes that are associated with rice


plants. On the other hand, production of cellulolytic and pectinolytic enzymes are of


major concerns along with the production of glagellins. Majority of other enzyme


also involved in these interactions in terms of degradation of reactive oxygen


species (ROS). Further, receptors and transporters for iron uptake also play sig-


nificant role in intracellular microbial interaction with plant through


quorum-sensing systems(QS). Several degradative pathways of plant metabolites


and various plant growth-promoting and biocontrol traits such as ACC-deaminase


activity, biological nitrogenfixation (BNF) and production of phytohormones and


volatile and non-volatile antimicrobial compounds.


Applying postgenomic approaches, such as metaproteomisa, metaproteoge-


nomics and metatranscriptomics, can link genomic potential with plant–endophyte


interactions. Recently, a metaproteogenomic approach was used to study the


microbial communities in the phyllosphere and rhizosphere of rice (Knief et al.


2011 ).


7.6 Plant Growth-Promoting Endophytes


Some of the endophytes act as biocontrol agents while others increase plant growth


through the production of nutrients and minerals such as nitrogen, phosphate and


other nutrients. Infact, quite a few endophytic bacterial genera such as


7 Role of Bacterial Endophytes in Plant Disease Control 141

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