Endophytes Crop Productivity and Protection Volume 2 (Sustainable Development and Biodiversity)

(Tina Meador) #1

P.fluorescensstrain (PDY7) was highly effective in reducing the incidence of


bacterial blight of rice (58.83 and 51.88%) under glass house andfield condition.


This is mainly due to the production of antibiotics called 2,4-diacetylphloroglucinol


(DAPG) (Velusamy et al. 2013 ). Twenty six bacterial strains isolated from leaf, root


and stem region of mangrove plant (Rhizopora mucronata). Among these, highest


number bacterial isolates from leaf (38.5%) followed by root (34.5%) and stem


(26.9%). Of these,five bacterial strains namelySerratia, Bacillus, Pseudomonas,


MicrococcusandEnterobacterexhibited broad-spectrum of antagonistic activity


against fungal and bacterial pathogen (Jose and Christy 2013 ). Among the bacterial


strains tested, strain MB04 and MB08 were highly inhibitory to the growth ofX.


campestris pv. oryzaecausing rice bacterial blight (Yuliar 2014 ).


The endophytic bacteria isolated from tomato plants tested against bacterial wilt


pathogen. Of the isolates tested, only Ps1 and Ps8 can inhibitR. solanacearum


in vitro using seed coat method. In in vivo test, 30 days old tomato seedlings were


soaked with endophytic bacteria showed 8.07–9.19% disease suppression within


15 – 16 days incubation period (Purnawati et al. 2014 ). Cotton seeds treated with


endophytic bacteria strainsB. subtilisUFLA285 recorded the lowest bacteria blight


incidence of 26% (de Medeiros et al. 2015 ). Four endophytic bacteria isolated from


potato stem tissue and it was tested against the growth ofStreptomyces scabiesin


agar plate method. The results revealed that all the isolates were highly inhibitory to
the growth of test pathogen (Flatley et al. 2015 ).


7.8 Conclusion


Plant pathogenic fungi particularly Pythium, Phytophthora, Sclerospora,


Rhizoctonia, PeronosclerosporaandPlasmoparacause enormous crop losses. At


present, fungicides are the only source to control plant diseases but they have


several disadvantages that (i) many of them are toxic to human being and animals,


and (ii) that develop further resistance to the pathogen. Several important questions


remain unanswered concerning the practical use of endophyte‘supplements’in


agriculture. However, with the correct management, they hold potential for the


control of current and emerging pathogens, as well as biotic stresses, as we


encounter deviation in these through climate change. This is likely to be achieved


through a better understanding of signalling between the host plant and the


microbiome, and, ultimately, the manipulation of root exudation profiles to recruit a


more beneficial root microbiome, of which the endosphere is an integral part. The


quality of these BCAs can be further increased by using fundamental knowledge to


improve methods for their production and to increase their shelf life.


7 Role of Bacterial Endophytes in Plant Disease Control 151

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