Endophytes Crop Productivity and Protection Volume 2 (Sustainable Development and Biodiversity)

Table 8.1

(continued)

Crop

Planttissue

Sterilization methodemployed

Strategy of planttissue inoculation

Isolation medium

Antimicrobialsupplementedto growthmedium

Number ofrecoveredendophyticactinobacterialisolates

Identi

fication of

endophyticactinobacterial isolates

References

Potato plants(SoIanumtuberosum

cv

Desir

é)

Rootsand stems

Sodiumhypochlorite 1%supplemented withTween-20 0.02% for1 minEthanol 70% for1 min3 times washing inthiosulfate/Ringer

’s

solution for 1 minIncubation ofgrounded materialwith 120 mMsodium phosphate(pH 8) for 2 h

Cut into pieces ofca. 0.3 cm andmaceratedIncubated for 2 h atroom temperaturewith sodiumphosphate (pH 8)120 mM

TSA (0.05x)R2A agar

Cycloheximide(100

μg/mL)

N.i.

Nocardia globerulaCorynebacteriumaquaticum

Garbeva et al.(^2001

)

Canola (

Brassica

napus

cv.

Westar)

Roots

Ethanol 95% for1 minAcidi

fied mercuric

chloride 0.1% (w/v)for 1 minWashed 10 timeswith sterile tapwater

Roots suspended in1/10 PBS,macerated andserially diluted

Trypticase soy broth0.3% (TSB) with 1.5%agar

None

65

Arthrobacter

spp.

Curtobacter

spp.

Micrococcus

spp.

Rathayibacter

spp.

Germida et al.(^1998

)

(continued)

170 M.F. Carvalho et al.