Endophytes Crop Productivity and Protection Volume 2 (Sustainable Development and Biodiversity)

Table 8.1

(continued)

Crop

Planttissue

Sterilization methodemployed

Strategy of planttissue inoculation

Isolation medium

Antimicrobialsupplementedto growthmedium

Number ofrecoveredendophyticactinobacterialisolates

Identi

fication of

endophyticactinobacterial isolates

References

Fodderleguminousplants (

Vigna

unguiculata

and

Trifoliumalexandrinum

)

Rootsandnodules

Ethanol 70% for5 minSodiumhypochlorite 0.9%for 20 minWashed 3 timeswith sterile waterSodium bicarbonatesolution 10% for10 min

Macerated tissues

Starch casein agar

None

34

Streptomyces

spp.

Actinopolyspora

spp.

Saccharopolyspora

spp.

Micromonospora

spp.

Nocardia

spp.

Kaur et al.(^2015

)

Chinese cabbage(Brassicacampestris

L.)

Roots

Ethanol 90% for1 minSodiumhypochlorite 3.1%for 6 minWashed 3 timeswith sterile water

Cut into 1 cmfragments

Humic acid

• vitamin

agarCorn meal agar

Cycloheximide(50

lg/mL)

Nalidixic acid(10

lg/mL)

81

Microbispora

spp.

Streptomyces

spp.

Micromonospora

spp.

Nocardia

spp.

Verrucosispora

spp.

Nonomuraea

spp.

Actinomadura

spp.

Thermomonospora

spp.

Lee et al.(^2008

)

Rice(Oryza sativa

cv.

Qilisimiao andHuajingxian)

Leavesand roots

Ethanol 70% for5 minSodiumhypochlorite 0.87%for 15 minSodium bicarbonatesolution 10% for15 minRinsed three timeswith autoclaveddistilledwater

Cut into 1 cmfragments

1.2% S medium

Potassiumdichromate(25

lg/mL)

Nalidixic acid(15lg/mL)

274

Streptomyces

spp.

Streptoverticillium

spp.

Tian et al.(^2004

)

(continued)

8 Endophytic Actinobacteria for Sustainable Agricultural Applications 171

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