Endophytes Crop Productivity and Protection Volume 2 (Sustainable Development and Biodiversity)

Table 8.1

(continued)

Crop

Planttissue

Sterilization methodemployed

Strategy of planttissue inoculation

Isolation medium

Antimicrobialsupplementedto growthmedium

Number ofrecoveredendophyticactinobacterialisolates

Identi

fication of

endophyticactinobacterial isolates

References

Cucumber seeds(Cucumis sativusL. cv. Cheyenne)

Roots

Exposure topropylene oxidevapors for 1 h

Macerated tissues

Humic acid

• vitamin

agar

Cycloheximide(50

lg/mL)

Nystatin(50

lg/mL)

29

Actinoplanes

spp.

Micromonospora

spp.

Streptomyces

spp.

Microbispora

spp.

Streptosporangium

spp.

El-Tarabilyet al. (

2009

)

Rice (

Oryza

sativa

L. cv.

KDML 105)

Leaves,leafsheathes,stems androots

Ethanol 70% for1 minSodiumhypochlorite 3% for6 minFinal rinse in sterilereverseosmosis-treatedwater

Cut into 1 cmfragments

Mannitol mung beanyeast extract mineralsalt agarTap water

• yeast

extract agarHumic acid

• vitamin B

agarTSA

Nystatin100 IU/mL

116

Streptomyces

spp.

Microbispora

spp.

Kineococcus

spp.

Kampapongsaand Kaewkla(^2016

)

Ginger (

Zingiber

of

ficinale

)

Tumeric(Curcumadomestica

)

Leaves,stems androots

Rinsed in Tween 200.1% for 30 sSodiumhypochlorite 1% for5 minWashed in steriledistilled water for5 minEthanol 70% for5 minAir-dried in alaminar

flow

chamber

Cut into smallpieces of ca. 4



4mm

2

Humic acid

• vitamin

agar

Nystatin(100

lg/mL)

Cycloheximide(100

lg/mL)

97

Streptomyces

spp.

Microbispora

spp.

Nocardia

spp.

Micromonospora

spp.

Taechowisanet al. (

2003

)

N.i.

Not indicated

172 M.F. Carvalho et al.