Endophytes Crop Productivity and Protection Volume 2 (Sustainable Development and Biodiversity)

(Tina Meador) #1

has been an enhanced focus on exploring indigenous medicinal plants with


antidiabetic potential so that a low cost, safer, and effective alternative strategy to


insulin and other synthetic compounds for diabetes management can be realized.


Hypoglycemic effects of several plants used to treat diabetes are already known and


the underlying mechanisms of the observed affects are also being worked out (Patel


et al. 2012 ). Recently, Mohammed et al. ( 2016 ), in a comprehensive study, eval-


uated antidiabetic activity ofCoccinia grandisagainst streptozotocin-induced dia-


betic rats. Treatment of diabetic rats withCoccinia grandisleaf ethanolic extract


(500 mg/kg) for 21 days showed significant reduction in blood glucose level,


increase in body weight and serum insulin in a dose dependent manner. Diabetes


induced hypercholesteremia and hypertriglyceridemia, which can lead to secondary


complications, were also found to be significantly improved as a result of treatment.


Marked recovery was also recorded in liver and kidney function tests of diabetic


rats. The study demonstrated a strong antioxidant activity of the ethanolic leaf


extract of the plant. It should be pointed out here that oxidative stress is a natural


consequence of chronically elevated blood glucose level and thus, compliments


diabetes (Rahimi et al. 2005 ). Baldea et al. ( 2010 ) evaluated antidiabetic activity of


crude ethanolic extract of 17 Boreal forest medicinal plants by in vitro on Caco-2


human enterocylic cell lines and in vivo on normal rats by oral administration of


250 mg/kg body weight of extract. In in vitro experiments, of the 17 plants, 13
showed approximately 40% decreased glycaemia and another 2 plants showed


reduction in intestinal glucose absorption in rats. Similarly, antidiabetic activity of


Allium cepa,Allium sativum, andZingiber officinaleaqueous extract was evaluated


against alloxan-induced diabetic rats. All three plant extracts showed decrease in


blood glucose level in treated diabetic rats as compared to control rats (Eyo et al.


2011 ). A comprehensive list of studies of the last 5–6 years on antidiabetic activ-


ities of plant/plant extracts demonstrating the interest and scope in thefield is given


in Table10.3.


10.4.1 Antidiabetic Compounds from Fungal Endophytes


Dhankar et al. ( 2013 ) demonstrated the antidiabetic and hypolipidemic activity of


endophytic fungi,Aspergillussp. andPhomasp. isolated fromSalvadora oleoides


(Salvadoraceae). The study showed that 2,6-di-tert-butyl-p-cresol and Phenol,


2,6-bis [1,1-dimethylethyl]-4-methyl, isolated from the broth extract of endophytic


fungi, significantly reduce blood glucose level in glucose loaded, fasting and al-


loxan-induced diabetic Wistar albino rats. Bioactivity-guided fractionation of the


culturefiltrate of an endophytic fungus,Dendryphion nanum,isolated fromFicus


religiosayielded a compound herbarine 1 and its analogue herbaridine A2 showing


promising antidiabetic activities. Both of them were found to be naphthaquinones


(Mishra et al. 2013 ).


Berberine from several medicinal plants is a compound known for its cardio-
protective, antidiabetic, antibiotic, and antitumor roles (Sun et al. 2009 ). Recently, it


222 S.I. Mohammed et al.


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