180
Fig. 9.7 Individual
subculture of each type of
cell in an MF-medium
(Mesenchymal Stem Cell
Growth Medium) (Toyobo,
Osaka, Japan) to
proliferate the cells
(Phase-contrast
microscopy). (a) Stellate
cells. Fibroblast-like cells
in the primary culture
become stellate in shape
and possess slender
cytoplasmic processes and
have small lipid droplets in
the cytoplasm. (b)
Colony-forming unit.
Cobblestone-like
squamous cells in an
MF-medium form a
colony-forming unit
Fig. 9.8 CD44 on
cytoplasm of cells in the
human adult macula flava,
shown by
immunohistochemical
staining
K. Sato