© Springer Nature Singapore Pte Ltd. 2017 223
T. Tsuji (ed.), Organ Regeneration Based on Developmental Biology,
DOI 10.1007/978-981-10-3768-9_12
Chapter 12
Liver Regeneration Using Cultured Liver Bud
Keisuke Sekine, Takanori Takebe, and Hideki Taniguchi
Abstract Human induced pluripotent stem cells (iPSCs) have highly promising
applications in regenerative medicine, drug discovery, and disease modeling, as
well as in investigations of developmental biology. Although iPSC can theoretically
be differentiated into any cell type, further developments are required for applica-
tion to tissue replacement. In particular, most differentiation protocols produce fully
matured populations of single cell types in two-dimensional cultures.
Some tissues, including retinal pigment epithelium, comprise relatively simple
structures of single cell types and can be replaced by monocultures of matured cells.
However, gastrointestinal and liver tissues comprise multiple cell types in complex
architectures. Therefore, the lack of blood perfusion and three-dimensional interac-
tions between multiple cell types are fundamental obstacles to the application of
iPSC to regenerative medicine.
K. Sekine
Department of Regenerative Medicine, Yokohama City University Graduate School
of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan
e-mail: [email protected]
T. Takebe
Department of Regenerative Medicine, Yokohama City University Graduate School
of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan
Advanced Medical Research Center, Yokohama City University,
3-9 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan
PRESTO, Japan Science and Technology Agency,
4-1-8, Honcho, Kawaguchi, Saitama 332-0012, Japan
Department of Pediatrics, Cincinnati Children’s Hospital Medical Center,
University of Cincinnati, 3333 Burnet Avenue, Cincinnati, OH 45229-3039, USA
e-mail: [email protected]; [email protected]
H. Taniguchi (*)
Department of Regenerative Medicine, Yokohama City University Graduate School
of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan
Advanced Medical Research Center, Yokohama City University,
3-9 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan
e-mail: [email protected]