Organ Regeneration Based on Developmental Biology

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Fig. 2.6 Self-formation of stratified cerebellar neuroepithelial structure. (a–d) Immunostaining of
Fgf19-treated aggregates on day 55. PCPZ, Purkinje cell precursor zone; VZ, ventricular zone.
(e–g) Self-formation of neuroepithelial structure in hESC aggregate. Immunostaining for PKCζ
(aPKC) and N-cadherin. (h) Immunostaining of SDF-1-treated aggregates on day 35. (i) Laminar
structures found in hESC-derived cerebellar neuroepithelium. RLZ, RL-derivative zone. (j–o)
Immunostaining on day 35 for cerebellar precursor-specific markers. The scale bars represent 200
μm (e–g), 100 μm (a and h) and 50 μm (d and apply to b and c), and 25 μm (o and apply to j–n)
(Modified from Muguruma et al. 2015 )

2 Self-Organized Cerebellar Tissue fromfiHuman Pluripotent Stem Cells andfiIts...