50
Fig. 3.3 Functional tests of mouse ES-derived ACTH+ cells. (a) In vitro release from mouse
ES-derived ACTH+ cells. “F” = glucocorticoid pretreatment. Among the releasing factors, CRH
most efficiently induces ACTH secretion. (b) Negative feedback test. Pretreatment with hydrocor-
tisone suppresses CRH-stimulated ACTH secretion from aggregates. (c) In vivo functional test by
ectopic transplantation. All mice, except for the WT mice, received a hypophysectomy; hypopitu-
itarism was confirmed by CRH loading. “S + D+” =SAG- and DAPT-treated aggregates. “S−D−”
= no SAG or DAPT treatment. The values shown on graphs represent mean ± s.e.m. P < 0.05,
P < 0.01, and P < 0.001 (from Suga et al., 2011 , modified). (d) Blood ACTH and subsequent
release of corticosterone. (e) Improved activity and survival
H. Suga and C. Ozone