Organ Regeneration Based on Developmental Biology

53

or small molecule agonists. However, ectopic transplantation is not perfect, because
physiological CRH released from the hypothalamus does not directly affect these
grafts. Orthotopic transplantation of hormone-producing cells that are controlled by
positive and negative regulators is one of the future candidates for complete
therapy.

b

a

Rx::venus Lim3

Cytokeratin

c

2um

2um

Rx::venus Lim3

Cytokeratin

Rx::venus Lim3

Cytokeratin

k

d70

ACTH

Tbx19

Lim3 ACTH

Lim3

Tbx19

ACTH

Pit1 GH

PRL

TSH

d67 d67

TSH

d84 +DX

LH

d82

FSH

d82

d85

GH

PITX1

+DX

d84

PRL

PITX1

+DX

Gata2

LH/FSH

Glucocorticoid

(dexamethasone;DX)

Fig. 3.4 Human ES culture (a) Recapitulation of Rathke’s pouch formation. (b) Differentiation
into multiple lineages. (c) Secretory granules characteristic of endocrine cells

3 Functional Pituitary Tissue Formation Recapitulating Hypothalamus andflPituitary...