On Biomimetics by Lilyana Pramatarova

On Biomimetics
82

(A) (B) (C)

Fig. 27. A: 3-D printed plaster femur head mold; B&C: Front and bottom view of Injected

PCL femur head.

4 days (n=3 wells for each cell in each kind of medium). The Alamar BlueTM assay was

performed every two days. The result shown in Figure 28 indicates that the 7F2 and EAhy

926 cells did not experience any significant cellular dysfunction due to the degradation

product of PCL scaffolds. ANOVA test for independent variables was used to check for

differences between results obtained for different mediums which showed no significant

change between the controls with the testing mediums.

4. Structured porogen method by a newly developed sucrose printing RP
   machine

The commercially available SFF systems we used previously are all using non-

biocompatible materials such as thermal plastic wax (Solidscpe Inc.), plaster (Z-corp),

photoresin (3D system Inc.) etc., and the porogen dissolving process is complicated and

time consuming. Most importantly, porogen materials cannot be completely cleaned up; and

their residues will stay with final scaffolds and will have negative effects on scaffold’s

bioactivities. To overcome the above mentioned problems we have designed and developed

a novel biomaterial-sucrose deposition system and used this system to fabricate structured

porogen.

Alamar Blue Assay for Toxicity Test

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

012345

Culture Time (Days)

Normalized Fluoresecence

7F2

7F2-CONTROL

EAhy 926

Eahy 926 CONTROL

Fig. 28. Toxicity testing result for PCL scaffolds.