87
specifi cities of members of the caspase family
and granzyme B. Functional relationships
established for key mediators of apoptosis.
J Biol Chem 272:17907–17911
- Stennicke HR, Deveraux QL, Humke EW et al
(1999) Caspase-9 can be activated without
proteolytic processing. J Biol Chem 274:
8359–8362
- Dorstyn L, Kumar S (2008) A biochemical
analysis of the activation of the Drosophila cas-
pase DRONC. Cell Death Differ 15:461–470
- Thornberry NA, Peterson EP, Zhao JJ et al
(1994) Inactivation of interleukin-1 beta con-
verting enzyme by peptide (acyloxy)methyl
ketones. Biochemistry 33:3934–3940
- Faleiro L, Kobayashi R, Fearnhead H et al
(1997) Multiple species of CPP32 and Mch2
are the major active caspases present in apop-
totic cells. EMBO J 16:2271–2281
- Read SH, Baliga BC, Ekert PG et al (2002) A
novel Apaf-1-independent putative caspase-2
activation complex. J Cell Biol 159:739–745
- Tu S, McStay GP, Boucher LM et al (2006) In
situ trapping of activated initiator caspases
reveals a role for caspase-2 in heat shock-
induced apoptosis. Nat Cell Biol 8:72–77
- Harvey NL, Butt AJ, Kumar S (1997)
Functional activation of Nedd2/ICH-1 (cas-
pase- 2) is an early process in apoptosis. J Biol
Chem 272:13134–13139
- Garcia-Calvo M, Peterson EP, Rasper DM et al
(1999) Purifi cation and catalytic properties of
human caspase family members. Cell Death
Differ 6:362–369
Assays for Studying Caspase-2
