139- When injecting two solutions, they can be injected separately.
However, care must be taken when injecting the same oocyte
twice not to cause too much damage to the oocyte. One sign
of this would be excessive leaking from the oocyte. Performing
the same number of injections in control oocytes will control
for any effect of the injections alone. - It is also possible to observe apoptotic activity in oocytes visu-
ally. Upon induction of apoptosis, X. laevis oocytes develop a
characteristic large, white spot in their dark half (animal pole)
(Fig. 4 ).
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Methods for the Study of Caspase Activation in the Xenopus laevis Oocyte...