Caspases,Paracaspases, and Metacaspases Methods and Protocols

(Wang) #1

Stokes’ shift ........................................................................3 1
Strong cation exchange chromatography (SCX) ..............2 57
Substrate specificity ..............................2 5–27, 41–58, 72, 73,
90, 99, 190, 205, 238, 242


T


TAP tag .................................................................... 2 24, 233
tBID ......................................................... 1 23, 131–132, 158
Terminal differentiation ............................................... 8 9, 90
Tobacco etch virus (TEV ) protease .............................. 3 6, 84
Transformation ................................................ 1 82, 193, 196,
205–206, 209
Trypsin ............................................... 6 2, 63, 65, 75, 81, 137,
194, 198, 200, 256, 258, 259
Tudor staphylococcal nuclease .......................... 2 39, 248, 249


V


Vacuolar staining ...................................................... 2 31–232
Vectashield ............................................9 2, 97, 111–113, 115,
144, 150, 162, 167


W
Western blotting .................................9 4, 123, 133, 142–144,
146–148, 151, 152, 156, 157, 159, 161, 165–166,
179, 181–185, 195–196, 199–200, 247, 249, 250
X
X chromosome-linked inhibitors of apoptosis (xIAPs) ....1 51
X-ray crystallography ........................................................2 04
Y
Yeast transformation .........................................................1 96
Yellow fluorescent protein (YFP) ........8 , 11, 12, 29, 142, 186
Z
Z-VAD-fmk ...................................................... 7 , 18–21, 25,
32, 33, 76, 79, 81, 160, 164, 191, 192, 194, 198
Z-VRPR-FMK .................................1 80, 186, 205, 209, 218
Zymogen .................................... 8 , 25–27, 29, 71, 72, 74, 84,
96, 107, 155–157, 166, 238, 239, 242, 243, 248

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CASPASES, PARACASPASES, AND METACASPASES: METHODS AND PROTOCOLS
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