Caspases,Paracaspases, and Metacaspases Methods and Protocols

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Fig. 5 The pattern of P4-P2 SCL spotting on the 96-well plate. This pattern is an alternative to the one
presented in Fig. 3

Fig. 6 Selection of optimal amino acids in P4-P2 positions from PS-SCL screening

Fig. 7 Scheme for synthesis of Ac-DEVD-ACC. The numbers from 1 to 12 indicate successive steps of the
synthesis. ( 1 ) Fmoc-ACC-OH coupling to the resin; ( 2 , 4 , 6 , 8 , and 10 ) Fmoc group deprotection; ( 3 ) Coupling
the fi rst amino acid to the NH 2 -ACC-resin; ( 5 , 7 , and 9 ) peptide chain elongation; ( 11 ) acetylation of NH 2 -
tetrapeptide- ACC; ( 12 ) substrate cleavage from the resin (removing protecting groups from the substrate) and
substrate precipitation

Combinatorial Methods to Defi ne Caspase Substrate Specifi city