very small values ofn, even small errors lead to very large errors in 1/n; this is
not noticeable at large values ofn(Dowd and Riggs, 1965). Weighting of the
data can help somewhat to overcome these shortcomings.
Another type of graphical plot used to analyze kinetic data is the Hanes–
Woolf plot (Fig. 4.3). In this case, [S]/nis plotted along they-axis and [S] is
plotted along thex-axis. For this plot, thex-intercept =Km, they-intercept
=Km/Vmaxand the slope of the best-fit line estimates 1/Vmax. The Hanes–Woolf
FIGURE 4.2 Lineweaver–Burk (double-reciprocal) plot of a reaction that follows
Michaelis–Menten kinetics. Kinetic parameters are as defined previously.
FIGURE 4.3 Hanes–Woolf plot of a reaction that follows Michaelis–Menten kinetics.
Kinetic parameters are as defined previously.
GRAPHICAL KINETIC PLOTS 93