FIGURE 10.2 Metabolite profiles of buspirone determined by HPLC with MSC, LSC,
and RFD (Zhu et al., 2005b). Buspirone metabolites from human liver microsome
incubations were separated by HPLC (1 mL/min). Radioactivity in HPLC effluent was
determined by; (a) TopCount: 8000 DPM injected, four fractions per min and 10 min
counting time; (b) LSC: 8000 DPM injected, two fractions per min, 10 min counting
time; and (c) RFD: 32,000 DPM injected.
292 APPLICATIONS OF LIQUID RADIOCHROMATOGRAPHY TECHNIQUES