Drug Metabolism in Drug Design and Development Basic Concepts and Practice

LC-SPE-MS-NMR HPLC system connected to a solid

phase extraction (SPE) system

Fraction of interest is

concentrated in thecartridge prior to the NMRanalysis

Not all compounds can be

trapped into the SPE cartridge,limiting its use with certaintype of compounds

Compound of interest is

‘‘trapped’’ in a SPE cartridgeand later transferred into theNMR flow probe for analysis

Chromatography can be

performed using protonatedsolvents with any modifier

Complex setup. Maintenance of

the hardware is time consuming

MS can be used during the LC

analysis and during the analysisof the cartridge

Sample is loaded into the NMR

flow cell using deuteratedsolvents minimizing the needfor solvent suppression

Various solid phase options

available in SPE cartridge tooptimize for differentcompounds

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