are the same as that described in Section 14.1.2 (the protocol for detection
of glutathione/N-acetylcysteine or cyano adducts using ion-trap mass
spectrometer).
14.1.3.2 Instrumentation LC/MS/MS is carried out on a Perkin-Elmer
SCIEX API 3000 tandem mass spectrometer (Toronto, Canada) interfaced to
FIGURE 14.4 LC/MS/MS analysis of theN-acetylcysteine adductDof (2S,3R)-(+)-3-
(3-hydroxyphenyl)-2-[4-(2-pyrrolidin-1-ylethoxy)-phenyl]-2,3-dihydro-1,4-benzoxathiin-6-
ol (C). (a) Total ion chromatogram of the mass scanning atm/z611. (b) ESI–MS spectrum
of the adduct D at 16.7 min. (candd) ESI–MS–MS spectra of the adductDobtained by
CID of the MH+ion atm/z611 (MS^2 )and464(MS^3 ), respectively. The origin of
characteristic fragment ions is as noted.
456 PROTOCOLS FOR ASSESSMENT