XVIII Color Plates
Figure 3B(Chapter 4)
Figure 6(Chapter 5). Ester substrate insn-1 orientation inRhizopus oryzaelipase (ROL, light gray)
andRhizomucor mieheilipase (RML, dark gray) (Scheib et al., 1999). The position of the G266 varies in
both lipases (Ca–Cadistance¼1.3 A ̊) due to the different position of E265 in ROL and T265 in RML,
respectively (horizontal arrow). Therefore, in ROL, the substrate binds deeper into the binding site than in
RML (C2RML–C2ROL¼0.9 A ̊). Hence, steric interactions between thesn-2 substituent and L258 are more
pronounced in ROL rather than RML (vertical arrow).