Front Matter

12.2 Sources of phospholipases 223

Table 1.Sources of phospholipases used in biocatalysis.

Phospholipase
(EC number)

Source Molecular

mass (kDa)

pI pH optimum Reference

PLA 2 (3.1.1.4) Porcine pancreas 13.9 7.4 7.9–8.4 De Haas et al., 1968

Bovine pancreas 14.0 6.4; 7.6 8.5; 8.0 Dutilh et al., 1975

Cobra venom

(Naja naja naja)

13.4 5.1 7–9 Reynolds and Den-

nis,1991

Bee venom

(Apis mellifica)

19.0 10.5 8.0 Shipolini et al., 1971

PLC (3.1.4.3) Bacillus cereus 23.0 6.6–8.0 Little, 1981

Clostridium

perfringens

43.0 5.4 Takahashi et al.,

1981; Krug and Kent,

1984

PI-PLC (3.1.4.10) Bacillus cereus 29.0 5.4 7.2–7.5 Ikezawa and
Taguchi, 1981

PLD (3.1.4.4) Cabbage 87.0 4.7 5.5–5.7
(at 40 mM
CaCl 2 )

Lambrecht and Ulb-

rich-Hofmann, 1992;

Lambrecht and Ulb-

rich-Hofmann, 1993

90.2 4.7 5.5 (at 45 mM

CaCl 2 );

7.5 (at 10 mM

CaCl 2 )

Abousalham et al.,

1993

91.8 (PLD1)

92.1 (PLD2)

Pannenberg et al.,

1998

Streptomyces

chromofuscus

50–57 5.1 8 Imamura and

Horiuti, 1979

Streptomyces

hachijoensis

16 8.6 7.5 Okawa and

Yamaguchi, 1975

Streptomyces

lydicus

56 7.4 6 Shimbo et al., 1990

Streptomyces sp.

(Asali Kasai;

Sigma)

46 4.2 5.5 Asali Kasei Enzymes

catalog, T-39, 1992

Streptomyces

antibioticus

64.0 6.5 5.5 Shimbo et al., 1993

Streptomyces

PMF

53.864 9.1 4–6 Carrea et al., 1995

Streptomyces

PM43

54.147 9.1 6–7 Carrea et al., 1995

Streptomyces

PMR

52.672 9.2 4–7 Carrea et al., 1997b

Streptoverticillium

cinnamoneum

18.2 8.44 8.5 Streikuviene et al.,

1986

53.879 6.0 Ogino et al., 1999