Front Matter

(Tina Sui) #1
240 12 Phospholipases Used in Lipid Transformations

Table 2.Fatty acids introduced by PLA 2 into glycerophospholipids.


Fatty
acid introduced


Educt Source of PLA 2 Solvent system Reference

[^3 H]-Oleic acid lyso-PC (egg yolk) Naja najaand
bee venom, porcine
and bovine pancreas,
Streptomyces
violoceoruber


toluene, benzene Pernas et al., 1990

Eicosapentanoic
(C20 : 5) and
docosahexanoic
(C22 : 6) acids


lyso-PC porcine pancreas buffer/isooctane,
AOT^1
(microemulsion)

Na et al., 1990

Saturated and
unsaturated
fatty acids


glycerophos-
pholipids

bee or snake
venom, porcine
pancreas (PLA 2
immobilized)

hexane, heptane,
petroleum ether,
chlorinated
hydrocarbons

Pedersen, 1991

Palmitic acid lyso-PC cobra venom
(Naja naja)


chloroform
(1 % water)

Lin et al., 1993

Saturated and
unsaturated
fatty acids


PC (egg yolk)
lyso-PC (egg yolk)
and others

porcine pancreas chloroform
and other

Mingarro et al., 1994

Polyunsaturated
fatty acids


lyso-PC
(egg yolk)

porcine pancreas
(PLA 2
immobilized)

isooctane,
ethanol

Lilja-Hallberg and
Ha ̈rro ̈d, 1994;
Lilja-Hallberg and
Ha ̈rro ̈d, 1995

Polyunsaturated
fatty acids


lyso-PC
(egg yolk)

porcine pancreas
(PLA 2
immobilized)

isooctane, CO 2
propanol (in a
high-pressure
reactor, under
controlled water
activity)

Ha ̈rro ̈d and Elfman,
1995

Eicosapentanoic
(C20 : 5) and
docosahexanoic
(C22 : 6) acids


lyso-PC
(soybean)

porcine pancreas glycerol,
formamide

Hosokawa et al.,
1995

Oleic acid and
other fatty acids


lyso-PC
(egg yolk)

porcine pancreas
(PLA 2
immobilized)

toluene
(under controlled
water activity)

Egger et al., 1997

(^1) AOT,bis(2-ethylhexyl)-sulfosuccinate.

Free download pdf