240 12 Phospholipases Used in Lipid Transformations
Table 2.Fatty acids introduced by PLA 2 into glycerophospholipids.
Fatty
acid introduced
Educt Source of PLA 2 Solvent system Reference
[^3 H]-Oleic acid lyso-PC (egg yolk) Naja najaand
bee venom, porcine
and bovine pancreas,
Streptomyces
violoceoruber
toluene, benzene Pernas et al., 1990
Eicosapentanoic
(C20 : 5) and
docosahexanoic
(C22 : 6) acids
lyso-PC porcine pancreas buffer/isooctane,
AOT^1
(microemulsion)
Na et al., 1990
Saturated and
unsaturated
fatty acids
glycerophos-
pholipids
bee or snake
venom, porcine
pancreas (PLA 2
immobilized)
hexane, heptane,
petroleum ether,
chlorinated
hydrocarbons
Pedersen, 1991
Palmitic acid lyso-PC cobra venom
(Naja naja)
chloroform
(1 % water)
Lin et al., 1993
Saturated and
unsaturated
fatty acids
PC (egg yolk)
lyso-PC (egg yolk)
and others
porcine pancreas chloroform
and other
Mingarro et al., 1994
Polyunsaturated
fatty acids
lyso-PC
(egg yolk)
porcine pancreas
(PLA 2
immobilized)
isooctane,
ethanol
Lilja-Hallberg and
Ha ̈rro ̈d, 1994;
Lilja-Hallberg and
Ha ̈rro ̈d, 1995
Polyunsaturated
fatty acids
lyso-PC
(egg yolk)
porcine pancreas
(PLA 2
immobilized)
isooctane, CO 2
propanol (in a
high-pressure
reactor, under
controlled water
activity)
Ha ̈rro ̈d and Elfman,
1995
Eicosapentanoic
(C20 : 5) and
docosahexanoic
(C22 : 6) acids
lyso-PC
(soybean)
porcine pancreas glycerol,
formamide
Hosokawa et al.,
1995
Oleic acid and
other fatty acids
lyso-PC
(egg yolk)
porcine pancreas
(PLA 2
immobilized)
toluene
(under controlled
water activity)
Egger et al., 1997
(^1) AOT,bis(2-ethylhexyl)-sulfosuccinate.