240 12 Phospholipases Used in Lipid Transformations
Table 2.Fatty acids introduced by PLA 2 into glycerophospholipids.
Fatty
acid introduced
Educt Source of PLA 2 Solvent system Reference[^3 H]-Oleic acid lyso-PC (egg yolk) Naja najaand
bee venom, porcine
and bovine pancreas,
Streptomyces
violoceoruber
toluene, benzene Pernas et al., 1990Eicosapentanoic
(C20 : 5) and
docosahexanoic
(C22 : 6) acids
lyso-PC porcine pancreas buffer/isooctane,
AOT^1
(microemulsion)Na et al., 1990Saturated and
unsaturated
fatty acids
glycerophos-
pholipidsbee or snake
venom, porcine
pancreas (PLA 2
immobilized)hexane, heptane,
petroleum ether,
chlorinated
hydrocarbonsPedersen, 1991Palmitic acid lyso-PC cobra venom
(Naja naja)
chloroform
(1 % water)Lin et al., 1993Saturated and
unsaturated
fatty acids
PC (egg yolk)
lyso-PC (egg yolk)
and othersporcine pancreas chloroform
and otherMingarro et al., 1994Polyunsaturated
fatty acids
lyso-PC
(egg yolk)porcine pancreas
(PLA 2
immobilized)isooctane,
ethanolLilja-Hallberg and
Ha ̈rro ̈d, 1994;
Lilja-Hallberg and
Ha ̈rro ̈d, 1995Polyunsaturated
fatty acids
lyso-PC
(egg yolk)porcine pancreas
(PLA 2
immobilized)isooctane, CO 2
propanol (in a
high-pressure
reactor, under
controlled water
activity)Ha ̈rro ̈d and Elfman,
1995Eicosapentanoic
(C20 : 5) and
docosahexanoic
(C22 : 6) acids
lyso-PC
(soybean)porcine pancreas glycerol,
formamideHosokawa et al.,
1995Oleic acid and
other fatty acids
lyso-PC
(egg yolk)porcine pancreas
(PLA 2
immobilized)toluene
(under controlled
water activity)Egger et al., 1997(^1) AOT,bis(2-ethylhexyl)-sulfosuccinate.