12 1 The Exploitation of Lipase Selectivities for the Production of Acylglycerols
Table 4.
Typical data for diglyceride production by lipase-catalyzed processes.
Process
Lipase
T(^8
C)
Yield
1
(%)
Majorisomer
Product composition
2
(% w/w)
Reference
FFA
MG
DG
TG
Substrate: triglyceridesHydrolysis
Porcine pancreatic lipase 40
67
1,2(2,3) 18
3
30
49
Plou et al., 1996
Penicillium
sp.
42
5
31
28
36
Gulomova et al., 1996
P. roquefortii
(solvent)
25
75
1,2(2,3)
Millqvist Fureby et al., 1997
Ethanolysis
P. roquefortii
(solvent)
25
75
1,2(2,3)
Millqvist Fureby et al., 1997
Directed glycerolysis
Pseudomonas
sp.
60–48
87
1,3
1
3
87
9
Yamane et al., 1994
Pseudomonas fluorescens
60–40 75–92 1,3
1
22
75
2
McNeill and Berger, 1993
Substrate: free fatty acids/glycerol1,3-Selective
Rhizomucor miehei
40
70–85 1,3
2–21 6–16
61–85 1–8 Rosu et al., 1999
esterification
Rhizopus arrhizus
40
70
n.a.
21
79
<
1
Millqvist Fureby et al., 1996
R. arrhizus
(directed)
40
82
18
5
77
0
Weiss, 1990
P. camembertii
(directed)
3
40
>
98
<
11
>
98
0
Weiss, 1990
Acylglycerol specific ester-ification
Penicillium camembertii
4
40
60
n.d.
40
25
35
Yamaguchi and Mase, 1991
1
Maximum yield on molar basis.
2
Product composition on weight basis, recalculated from experimental data reported in literature.
3
Molar ratio glycerol/fatty acid 3:1 to 4:1.
4
20% (w/w) water in glycerol.n.a., not available; n.d., not detected.
