10 kJ mol–1(Buchholz and Ru ̈th, 1976; Lasch, 1979). More detailed reports on this
theme have been published by Bisswanger (1994), Lasch (1979) and Korus and
O’Driscoll (1974), and with many practical examples by Buchholz and Kasche
(1997).
13.4 Immobilization of phospholipases
Compared to corresponding publications on lipases, the number of reports dealing
with the immobilization of phospholipases is small. Research has mainly been car-
ried out based on aspects of the application of immobilized phospholipases for ana-
lytical purposes. Other contributions attend to the possibility of the immobilization
13.4 Immobilization of phospholipases 273
Figure 5. (A) The activity of urease immobilized by entrapment within spherical Ca-alginate beads
versus particle diameter. The activity is inversely proportional to the particle size. (B) The activity of
urease entrapped within Ca-alginate increases with increasing amount of enzyme supply. However,
due to diffusion limitation the specific activity decreases markedly.
(A)
(B)