Front Matter

2.2 Fractionation of fatty acids and other lipids by lipase-catalyzed reactions 27

Table 1.Specificity of lipases in view of kinetic resolution of fatty acids and other lipids.

Lipase from Reaction Specificity Reference

Porcine pancreas Hydrolysis of whale
oil triacylglycerols

Discrimination against

n-3 22 : 6 as compared

to C 16 and C 18 acyl

moieties

Bottino et al., 1967

Porcine pancreas Hydrolysis of seed
oil triacylglycerols

Discrimination against

acyl moieties having

atrans-3 olefinic bond

Kleiman et al., 1970

G. candidum Hydrolysis of triacyl-
glycerols containing
isomericcis-octadece-
noyl moieties

Strong preference for

cis-9–18 : 1

Jensen et al., 1972

Porcine pancreas Hydrolysis of triacyl-
glycerols containing
isomericcis-octadecen-
oyl moieties

Discrimination against

cis-2-18 : 1 tocis-7-18 : 1

Heimermann et al., 1973

G. candidum Hydrolysis of triacyl-
glycerols

Distinct preference for

C 18 acyl moieties

havingcis-9- orcis-9,

cis-12-bonds

Jensen, 1974

G. candidum Hydrolysis of triacyl-
glycerols

Distinct preference for

C 18 acyl moieties havingcis-9-

orcis-9,cis-12-bonds and dis-

crimination againstcis-13-22 : 1

Baillargeon and Sonnet,

1991

G. candidum
(lipase B)

Hydrolysis of fatty acid

methyl esters

Highly selective for

cis-9–18 : 1 as compared

tocis-11-18 : 1 or

trans-9-18 : 1

Sidebottom et al., 1991

Oat (Avena sativa)
seed lipase

Hydrolysis of triacyl-

glycerols

Discrimination against

tripetroselinin as compared to

triolein, trilinolein

and tri-a-linolenin

Piazza et al., 1992

G. candidum Esterification of fatty
acids withn-butanol

Distinct preference for

C 18 acyl moieties havingcis-9-

orcis-9,cis-12-bonds and discri-

mination againstcis-13-22 : 1,

trans-9-18 : 1, n-6 18 : 3, ricino-

leic and 9-docosynoic acids

Sonnet et al., 1993b

G. candidum
(lipase B)

Hydrolysis of triacyl-

glycerols and esterifi-

cation of fatty acids

withn-octanol

Highly selective for

triolein and forcis-9-18 : 1

as compared tocis-6-18 : 1,

cis-11–18:1 ortrans-9-18 : 1

Charton and Macrae,

1993

Pythium ultimum Hydrolysis of triacyl-
glycerols

Preference for tri-a-

linolenin>trilinolein

>triolein

Mozaffar and Weete,

1993

Oat caryopses Hydrolysis of milk
fat triacylglycerols

Preferential hydrolysis

of C 6 -C 10 acids

Parmar and Hammond,

1994