Front Matter

28 2 Fractionation of Fatty Acids and Other Lipids Using Lipases

Table 1.continued

Lipase from Reaction Specificity Reference

R. miehei Alcoholysis of fatty
acid ethyl esters with
n-propanol

Preference forcis-11-20 : 1

andcis-13-22 : 1, strong

discrimination against

n-3 22 : 6, and moderate

discrimination against n-3 20 : 5

Bech Pedersen and Hol-

mer, 1995

Candida
parapsilosis

Hydrolysis of fatty

acid methyl esters

Substrate preference:

cis-9–16 : 1cis-9-18 : 1>;

n-6 18 : 3>cis-11-18 : 1

>cis-6-18 : 1

Briand et al., 1995

C. rugosa Hydrolysis of
borage oil

Discrimination against

n-6 18 : 3

Ergan, 1996

R. miehei Hydrolysis of fatty
acid ethyl esters

Discrimination against

n-3 22 : 6 as compared

to n-6 18 : 2 and n-3 18 : 3

Kosugi et al., 1997

Flavobacterium
odoratum

Hydrolysis ofp-nitro-

phenyl esters of fatty

acids

Substrate preference:

n-6 18 : 2>cis-9-18 : 1

n-6 18 : 3>cis-6-18 : 1

>trans-9-18 : 1

Labuschagne et al., 1997

R. delemar Interesterification of
randomized triacyl-
glycerols with
caprylic acid

Strong discrimination

against n-6 18 : 3 and

n-3 22 : 6; moderate

discrimination against

n-6 20 : 4 and n-3 20 : 5

Shimada et al., 1997b

C. rugosa,
Pseudomonas
cepacia, porcine
pancreas

Esterification of

acetylenic fatty acids

withn-butanol

Preference for 10-undecy-

noic acid and strong

discrimination against

6-octadecynoic acid

Lie Ken Jie and Xun,

1998a

C. rugosa,
R. miehei,
porcine pancreas

Esterification of

medium- and long-

chain olefinic and

acetylenic alcohols

with pentanoic acid

and stearic acid

Discrimination against

medium- and long-chain

olefinic alcohols in the esteri-

fication with pentanoic acid;

olefinic and acetylenic alcohols

well accepted in the esterification

with stearic acid catalyzed by

lipase from porcine pancreas

Lie Ken Jie and Xun,

1998b

Burkholderiasp. Hydrolysis oft-butyl
esters

Strong preference for

t-butyl octanoate as

compared tot-butyl

palmitate and stearate

Yeo et al., 1998

C. rugosa,
R. miehei,
C. antarctica
lipase A

Esterification ofcis-

andtrans-octadecenoic

acids withn-butanol

Preference for oleic acid

over elaidic acid by

C. cylindraceaand

R. miehei, whereas strong

preference for elaidic acid by

C. antarcticalipase A

Borgdorf and Warwel,

1999