Catalyzing Inquiry at the Interface of Computing and Biology

160 CATALYZING INQUIRY

acpM, which was not included in the interaction data used to construct the original, whole network)
showed up in the INH response network, all with significant up-regulation (Figure 5.10). Furthermore,
the specific removal of these genes (kasA, kasB, fabD, accD6) from the initial set of genes did not affect
their presence in the INH response subnetwork: the newly calculated network continued to contain
each of them. Forst concluded that INH does directly interfere with the FAS-II fatty acid production
pathway, in confirmation of earlier results.

Fatty Acid Degradation Network

Fatty acid degradation

Lipid biosynthesis

Neither

Cytoplasm

Cell wall

Membrane

Membrane and cell wall

Membrane and cytoplasm

FIGURE 5.9 Fatty acid degradation network. SOURCE: Courtesy of Christian Forst, Los Alamos National Labora-
tories, December 8, 2004.