A Practical Guide to Cancer Systems Biology

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  1. Protein Structure Modeling 123


Figure 6. Quality assessment with QMean server. (a) Job submission page and
(b) evaluation report pertaining information about overall quality of the model (black
box A) and local quality of the model (black box B).



  1. Quality assessment with ModEval sever


ModEval will give you the same scores as the ones used in ModWeb.


Materials



  • The model created by I-tasser or SwissModel. (Since models from ModWeb
    use the same scoring matrix as ModEval server, submitting them would
    be unnecessary)

  • Sequence identity between your query sequence and template sequence.
    For models obtained from SwissModel, open the model pdb file with a
    text editor, search for “SID” in ‘REMARK’ section (Fig. 7(a)).
    For models obtained from ModWeb, open the model pdb file with a text
    editor and search for “SEQUENCE IDENTITY” (Fig. 7(b)).


Beware that ModEval require an integer as sequence identity input.
Since I-tasser uses multiple templates in the process of model
construction, ModEval may not be suitable for evaluating I-tasser
models.


  • MODELLER License key. See ‘ModWeb’ section above for method of
    obtaining license key.

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