A Practical Guide to Cancer Systems Biology

48 A Practical Guide to Cancer Systems Biology

XIX. Elutepeptides by adding 20μLsolutionB, 1,000×gfor1minute.

XX. Transfer the eluted samples to a new 1.5 mL tube. Sample can be

stored at 20◦C for one month.

Hint: Post-fractionation of phosphopeptide samples can be per-

formed to increase the number of identified phosphopeptides

XXI. Vacuum dry the samples.

XXII. Dissolve peptides by 0.1 % TFA and subject to LC-MS/MS.

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