262 J.M. Seguí-Simarro et al.
Table 1
Putative components of the cell plate assembly matrix (CPAM)
Family
/Type
Structures
Candidate
Localization
Function
References
observed
molecule(s)
Plant exocyst
Y
/L-shaped
AtSec5, AtSec6, AtSec15, Golgi-derived
Vesicle tethering
(Cvrckova et al., 2001).
tethers
AtExo70, AtExo84
vesicles
Plant exocyst
Y-shaped tethers AtSec3
CPAM
Vesicle tethering
(Segu
́ı-Simarro et al., 2004).
Rab GTPases
—
???
CPAM matrix
Vesicle tethering
(Bednarek and Falbel, 2002;
and cell plate
Falbel et al., 2003).
Rops (plant
—
AtRop4, Rop1
CPAM matrix
Vesicle tethering, formation of
(Hong et al., 2001a;
Rho-GTPases)
and cell plate
short actin filaments, regulation
Molendijk et al., 2001;
of callose synthase complexes
Bednarek and Falbel, 2002).
Syntaxins
—
KNOLLE, SNAP33,
Fusing
Vesicle fusion
(Lauber et al., 1997;
NSPN11, KEULE
vesicles
Assaad et al., 2001;Zheng et al., 2002).
DRP1
Rings & springs
DRP1A,
Cell plate
Membrane squeezing,
(Gu and Verma, 1996, 1997;
phragmoplastin
intermediates
stretching and stabilization
Otegui et al., 2001;Kang et al., 2003;Segu
́ı-Simarro et al., 2004).
DRP2
Rings & springs
DRP2A
Clathrin-
Formation of recycling
(Verma, 2001;
coated buds
clathrin-coated vesicles
Konopka et al., 2006).
EB1
MT–cell
AtEB1, AtMAP65-3
CPAM-
Anchoring of MTs
(Mathur et al., 2003;
plate linkers
embedded
to the cell plate
Muller et al., 2004;
MT (+) ends
van Damme et al., 2004).
actin
Fine,
6
nm
F-actin
CPAM matrix
Multiple roles (see text)
(Endle et al., 1998;
filaments
and cell plate
Otegui et al., 2001;Bednarek and Falbel, 2002;Otegui and Staehelin, 2004).
Long coiled-
—
???
CPAM matrix
CPAM scaffold
Kang and Staehelin,
coil proteins
submitted