Figure 2. Development of the screen for dominant, apoptosis-inducing genes.
(a) The original version of the screen comprised the transfection of small plasmid pools of at
least 20 different genes and the further investigation of single clones from positive pools. (b)
The next format was implemented with 96-well plates, employing a special DNA isolation
protocol that yielded transfection-pure plasmid DNA. (c) The robotic version of the screen
uses a transfection robot tailored to the screen that allows researchers to perform a high-
throughput assay.
CELL-CULTURE SYSTEMS IN APOPTOSIS 211