Characterization of Anti-Coxsackie Virus B3 Constituents ... 157Table 2. Main phenolic compounds identified in subfractions from EtOAc
fraction of S. arenaria rootsN° (^) Compounds RT (min) % in SR 1 % in SR 2 % in SR 3 % in SR 4
1 Tannic acid 2.35 (^) ND ND ND 3.01
2 Catechin 3.59 (^) ND ND ND 1.11
3 Protocatechuic acid 3.95 ND ND ND 71.94
4 Resorcinol 4.19 (^) ND ND ND 5.35
5 Methyl gallate 5.39 ND ND ND 7.61
6 Vanillic acid 6.26 (^) ND ND ND 4
7 Unknown 7.32 (^) ND ND ND 6.92
8 Unknown 10.59 (^) ND ND 4.12 (^) ND
9 Trans- 4 - hydroxycinnamic acid 10.7 (^) ND ND 16.06 (^) ND
10 Taxifolin 10.89 ND ND 59.42 ND
11 Unknown 11.41 (^) ND ND 15.36 (^) ND
12 Naringin 11.48 (^) ND 9.25 (^) ND ND
13 Naringenin- 7 - O-glucoside 11.64 (^) ND 3.51 (^) ND ND
14 Rutin 12.01 ND 41.46 ND ND
15 a-methyl- 4 - hydroxybenzoate 12.31 (^) ND 12.49 5.01 (^) ND
16 Unknown 12.42 ND 1.41 ND ND
17 Morin 13.08 (^) ND 3.78 (^) ND ND
18 Hesperetin 14.71 18.09 (^) ND ND ND
19 Unknown 15.58 14.3 (^) ND ND ND
20 Unknown 15.92 64.87 (^) ND ND ND
21 Unknown 17.16 1.75 ND ND ND
22 Unknown 17.96 0.97 (^) ND ND ND
23 Unknown 23.35 ND 28.05 ND ND
RT: Retention times (minutes).
ND: Not detected.
CONCLUSION
As far as we are concerned, the present work constitutes the first report
studying the antiviral activity of Scabiosa arenaria Forssk. extracts against
coxsackie B3. Furthermore, the antiviral activity of roots EtOAc, water
fractions and the flowers butanol fraction showed the best activity. The roots
EtOAc fraction was then submitted to chromatographic separation process.