Endophytic Fungi: Diversity, Characterization and Biocontrol

Endophytic Fungi Isolated from Vochysia divergens ... 97

Filo Class Identification Fungal

isolate

Tree

Location

Soil

Characteristic

GenBank

access

number

Ascomycota Dothideomycetes Phyllosticta

capitalensis

LGMF1175 A Flooded JX559595

LGMF1155 D Flooded JX559576

LGMF1132 1 Dry JX559553

LGMF1180 A Flooded JX559600

LGMF1189 A Flooded JX559607

LGMF1163 C Dry JX559584

LGMF1160 C Dry JX559581

LGMF1149 E Dry JX559570

LGMF1176 A Flooded JX559596

LGMF1158 C Dry JX559579

LGMF1167 B Dry JX559587

LGMF1185 A Flooded JX559605

LGMF1170 A Flooded JX559590

LGMF1162 C Dry JX559583

LGMF1192 A Flooded JX559610

LGMF1184 A Flooded JX559604

LGMF1147 E Dry JX559568

LGMF1190 A Flooded JX559608

LGMF1171 A Flooded JX559591

LGMF1169 B Dry JX559589

LGMF1146 E Dry JX559567

LGMF1168 B Dry JX559588

LGMF1154 D Flooded JX559575

Phyllosticta sp. LGMF1193 A Flooded JX559611

LGMF1172 A Flooded JX559592

Phaeosphaeria sp. LGMF1195 A Dry JX559613

Neofusicoccum

grevilleae

LGMF1116 4 Flooded JX559538

Pseudofusicoccum

stromaticum

LGMF1122 2 Flooded JX559543

LGMF1118 3 Flooded JX559540

Cladosporium sp. LGMF1129 2 Flooded JX559550

Annellosympodiella

sp.

LGMF1117 3 Flooded JX559539

Note: Nine plant samples were collected on different occasions between 2008- 2011. The collection points were
identified by letters and numbers: 1(19.501944ºS/55.601389ºW), 2(19.500833ºS/55.600556ºW),
3(19.500556ºS/55.600278ºW), 4(19.501111ºS/55.600556ºW), A(19.508548ºS/55.628775ºW),
B(19.506286°S/55.610172ºW), C(19.509930ºS/55.627039ºW), D(19.509038ºS/55.626354ºW) and
E(19.507243ºS/55.615815ºW).

DNA Extraction, Amplification, Sequencing, and Phylogenetic

Analysis

To extract DNA, the isolates were cultured in PDA for 3 days at 25°C and
then subjected to a MOBIO^ Ultraclean Microbial DNA Isolation Kit (MO
Bio, Carlsbad, US), following the manufacturer’s instructions.