Endophytic Fungi: Diversity, Characterization and Biocontrol

(C. Jardin) #1
Endophytic Fungi Isolated from Vochysia divergens ... 101

stereomicroscope. For this experiment, we used a randomized design with
three replications.


RESULTS AND DISCUSSION


Medicinal plants are considered a repository of endophytic fungi, and the
quest to identify novel bioactives has led to the exploration of plants located in
places with unique ecological adaptations (Somaiah et al. 2014). To explore
biodiversity in the medicinal plant V. divergens found in the Pantanal, a
peculiar wetland in Brazil, 77 endophytic fungal strains were isolated. These
isolates were identified by phylogenetic analysis of the ITS region as strains
belonging to the phyla Basidiomycota (4%) and Ascomycota (96%).


Figure 2. Bayesian phylogenetic tree based on ITS sequence, showing the relationships


between Polyporus, Peniophora, Irpex and Antrodia genera. The tree is rooted with


Geastrum campestre sequence obtained from GenBank. Scale bar shows 8 changes and
Bayesian posterior probability values are indicated at the nodes.

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