AMPK Methods and Protocols

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PK

PH PK PKC

C1 C1C1C1 C2C1C 1 PK PKC

C1 C1C1C1 C2C1C^1 PK PKC

C1 C1C1C1 C2C1C 1 PK PKC

C1 C1C1C1 C2C1C 1 PK PKC

C1 C1C1C1 PK PKC

PH PK PKC

PH PK PKC

PH PK PKC

PK PKC

PK PKC

PK PKC

PK PKC

PH PK PKC

PK PKC PK

PK

DUF3543

DUF3543

MIT

MIT

MIT

MIT

MIT

MIT

DUF3543

DUF3543

DUF3543

PK

PKCPKC

DUF3543

PH PKCPKC

PK

PK PK

PK

DUF3543

Human RPS6K A PK PKC

Human RPS6K B

Fungi RPS6K B - like

Plants RPS6K B - like

Nemve RPS6K A

Nemve RPS6K B

Human AKT 1

Human AKT 2

Human AKT 3

Drome AKT 1/2/3

Human PRKC A

Human PRKC B

Human PRKC G

Drome PRKC

Fungi PRKC

Dicdy AKT-like

Human CAMKK 1

Human CAMKK 2

Drome CAMKK

Fungi CAMKK

Plants CAMKK

Human STK 11

Nemve STK 11

Fungi STK 11

Plants

Plants

Human PRKAA 1

Human PRKAA 2

Drome PRKAA

Fungi PRKAA

Plants PRKAA

Human MAPK 1

Human MAPK 3

Drome MAPK

Fungi MAPK

Plants MAPK

KA1

Human ULK 1

Human ULK 2

Drome ULK 1/2

Nemve ULK 1/2

Human ULK 3

Drome ULK 3

Nemve ULK 3

Fungi ULK 1/2 - like

Plants ULK 1/2 - like

LECA

1 2 3 4 5 6 7

1 2

3 - 6 7

gene loss

gene loss

Fig. 12A hypothetical evolutionary scenario of eukaryotic kinase evolution. The figure integrates the results
from phylogenetic tree reconstruction and Pfam domain architecture analysis. The phylogenetic relationships
of contemporary kinases imply the existence of seven proto-kinases in the last eukaryotic common ancestor
(LECA). A parsimony criterion was used to infer the Pfam domain architecture of these ancestral proteins
(numbers 1–7 below the tree).See[12] for details

Tracing AMPK Evolution 137