AMPK Methods and Protocols

they are heat shock, ribosomal and mitochondria proteins;

proteasome subunits, elongation factors, etc. The collection

of positive interactors obtained after the AMPK-based two-hy-

brid screening should be compared with this list to validate the

possible candidates.

Acknowledgements

This study was supported by grants from the Spanish Ministry of

Education and Science (SAF2014-54604-C3-1-R) and Generalitat

Valenciana (PrometeoII/2014/029) to P.S.

References

1. Carling D, Thornton C, Woods A, Sanders MJ
   (2012) AMP-activated protein kinase: new reg-
   ulation, new roles? Biochem J 445(1):11–27


2. Hardie DG (2014) AMP-activated protein
   kinase: maintaining energy homeostasis at the
   cellular and whole-body levels. Ann Rev Nutr
   34:31–55


3. Hardie DG, Ashford ML (2014) AMPK: reg-
   ulating energy balance at the cellular and whole
   body levels. Physiology (Bethesda) 29
   (2):99–107


4. Tuerk RD, Thali RF, Auchli Y, Rechsteiner H,
   Brunisholz RA, Schlattner U, Wallimann T,
   Neumann D (2007) New candidate targets of
   AMP-activated protein kinase in murine brain
   revealed by a novel multidimensional substrate-
   screen for protein kinases. J Proteome Res 6
   (8):3266–3277


5. Thali RF, Tuerk RD, Scholz R, Yoho-Auchli Y,
   Brunisholz RA, Neumann D (2010) Novel
   candidate substrates of AMP-activated protein
   kinase identified in red blood cell lysates. Bio-
   chem Biophys Res Commun 398(2):296–301


6. Behrends C, Sowa ME, Gygi SP, Harper JW
   (2010) Network organization of the human
   autophagy system. Nature 466(7302):68–76


7. Ewing RM, Chu P, Elisma F, Li H, Taylor P,
   Climie S, McBroom-Cerajewski L, Robinson
   MD, O’Connor L, Li M, Taylor R,
   Dharsee M, Ho Y, Heilbut A, Moore L,
   Zhang S, Ornatsky O, Bukhman YV,
   Ethier M, Sheng Y, Vasilescu J, Abu-Farha M,
   Lambert JP, Duewel HS, Stewart II, Kuehl B,
   Hogue K, Colwill K, Gladwish K, Muskat B,
   Kinach R, Adams SL, Moran MF, Morin GB,
   Topaloglou T, Figeys D (2007) Large-scale
   mapping of human protein-protein interac-
   tions by mass spectrometry. Mol Sys Biol 3:89
   8. Qi J, Gong J, Zhao T, Zhao J, Lam P, Ye J, Li
   JZ, Wu J, Zhou HM, Li P (2008) Downregu-
   lation of AMP-activated protein kinase by
   Cidea-mediated ubiquitination and degrada-
   tion in brown adipose tissue. EMBO J 27
   (11):1537–1548
   9. Bruckner A, Polge C, Lentze N, Auerbach D,
   Schlattner U (2009) Yeast two-hybrid, a pow-
   erful tool for systems biology. Int J Mol Sci 10
   (6):2763–2788


8. Solaz-Fuster MC, Gimeno-Alcaniz JV,
   Casado M, Sanz P (2006) TRIP6 transcrip-
   tional co-activator is a novel substrate of
   AMP-activated protein kinase. Cell Signal 18
   (10):1702–1712


9. Moreno D, Viana R, Sanz P (2009)
   Two-hybrid analysis identifies PSMD11, a
   non-ATPase subunit of the proteasome, as a
   novel interaction partner of AMP-activated
   protein kinase. Int J Biochem Cell Biol 41
   (12):2431–2439


10. Rolland T, Tasan M, Charloteaux B, Pevzner
    SJ, Zhong Q, Sahni N, Yi S, Lemmens I,
    Fontanillo C, Mosca R, Kamburov A, Ghias-
    sian SD, Yang X, Ghamsari L, Balcha D, Begg
    BE, Braun P, Brehme M, Broly MP, Carvunis
    AR, Convery-Zupan D, Corominas R,
    Coulombe-Huntington J, Dann E, Dreze M,
    Dricot A, Fan C, Franzosa E, Gebreab F,
    Gutierrez BJ, Hardy MF, Jin M, Kang S,
    Kiros R, Lin GN, Luck K, MacWilliams A,
    Menche J, Murray RR, Palagi A, Poulin MM,
    Rambout X, Rasla J, Reichert P, Romero V,
    Ruyssinck E, Sahalie JM, Scholz A, Shah AA,
    Sharma A, Shen Y, Spirohn K, Tam S, Tejeda
    AO, Trigg SA, Twizere JC, Vega K, Walsh J,
    Cusick ME, Xia Y, Barabasi AL, Iakoucheva
    LM, Aloy P, De Las Rivas J, Tavernier J, Cal-
    derwood MA, Hill DE, Hao T, Roth FP, Vidal

156 Pascual Sanz et al.