Fig. 2Screenshots from online CRISPR design tool. (A) Graphical representation of location for proposed
AMPKα1 sgRNA sequence withinPRKAA1gene. (B) Specificity scores for proposed AMPKα1 sgRNA sequence.
Each guide has a specificity score from 0 to 100. (C) Details about potential off-targets. CRISPR Design
application software is available athttp://crispr.mit.edu
178 Adrien Grenier et al.