- Reusable silicone sealing mat for PCR-plate(s) (Fig.1A).
- Homemade “sandwich device” to keep the silicone sealing mat
firmly in place when the PCR-plate(s) rotates end over end
during the IP procedure (Fig.1B).
Fig. 1Pictures showing the special equipment used for the medium throughput AMPK activity assay. (A) The
96-well PCR-plate in the aluminum block on ice with the silicone sealing mat drawn aside. (B) The “sandwich
device” with two flat bottom 96-well plates holding two 96-well PCR-plates with silicone sealing mats on top.
The “sandwich device” holds the assembly together, so it can rotate end over end without fluid leaking out of
the wells of the PCR-plate. (C) Polycarbonate suction device with eight pipette tips for discarding the wash
supernatant from the wells of the 96-well PCR-plate. The length of the pipette tips is adjusted so they cannot
reach the bottom 10μl of the wells, safeguarding the agarose beads from accidental disposal
218 Jesper B. Birk and Jørgen F. P. Wojtaszewski