AMPK Methods and Protocols

1.0

1.5

2.0

2.5

3.0

3.5

Relative AMPK activity(Normalized

to WT MEFs

expressing empty vector)

0.5

1.0

1.5

2.0

0.5

1.0

1.5

Diff

(Cyto)

Diff

(Nuc)

NES NLS PM Golgi ER Mito Lyso

constitutively active form of AMPK

DKO MEFs

Dominant negative form of AMPK

Relative AMPK activity

(Normalized to WT MEFs)

A

B

C

Relative AMPK activity(Normalized to WT MEFs expressing empty vector)

ABKAR

osABKAR

Fig. 6Dynamic range of ABKAR and osABKARs is shown. The dynamic range of each AMPK biosensor was
obtained from wild-type mouse embryonic fibroblasts (MEFs) expressing a constitutively active form of AMPK
(a), AMPKαsubunit double knockout MEFs (b), and wild-type MEFs expressing a dominant negative form of
AMPK (c) under nutrient-rich condition. Reproduced from [11] with permission

Genetically-Encoded Sensors and Impeders 271