Fig. 2AMPK activation inhibits phenylephrine-mediated increase in protein synthesis and underlying signal-
ing. (a–d) Neonatal rat ventricular cardiomyocytes (NRVMs) were treated with (open bars) or without (solid
bars) phenylephrine (PE) in the presence or absence of phenformin (1 mM) for 24 h. (a) Amino acid
incorporation into proteins measured by [^14 C]-phenylalanine incorporation. N¼4. (b) Representative
immunoblot of eEF2 (Thr56) phosphorylation state and total eEF2 content. (c–d) Representative immunoblot
and quantification of p70S6K (Thr389) phosphorylation state and total p70S6K content.N¼3. (e–f) NRVMs
were treated with (open bars) or without (solid bars) phenylephrine (PE) in the presence or absence of AICAr
(1 mM) for 24 h. (e) Amino acids incorporation into proteins measured by [^14 C]-phenylalanine incorporation.
N¼3. (f) Representative immunoblots of p70S6K (Thr389) and eEF2 (Thr56) phosphorylation states and their
respective total content. Data are means.e.m. and were analyzed using two-way ANOVA followed by
Bonferroni posttest (*p<0.05 vs. untreated cells,#p<0.05 vs. PE-treated cells)
332 Florence Mailleux et al.