- Fill plate wells with 20μl of each sample in duplicates in
96-well plate (seeNote 30). - Wash the luminometer injector with distillated water.
- Place the plate into the luminometer with injector. The injector
adds 100μl of luciferase assay reagent per well in by one; each
well is read 2 s after injection.
Fig. 3AMPK activation inhibits phenylephrine-induced cardiomyocyte hypertrophy. (a,b) NRVMs were treated
with (open bars) or without (solid bars) phenylephrine (PE) in the presence or absence of phenformin (1 mM)
for 24 h. (a) Representative images of cardiomyocytes afterα-actinin immunostaining. (b) Quantification of
cardiomyocyte area.N¼3. (c,d) NRVMs were treated with (open bars) or without (solid bars) phenylephrine
(PE) in the presence or absence of AICAr (1 mM) for 24 h. (c) Representative images of cardiomyocytes after
α-actinin immunostaining. (d) Quantification of cardiomyocyte area.N¼3. Scale bar, 20μm. Data are
means.e.m. and were analyzed using two-way ANOVA followed by Bonferroni posttest (*p<0.05
vs. untreated cells,#p<0.05 vs. PE-treated cells)
Study of Cardiac Hypertrophy and Protein Synthesis 337