AMPK Methods and Protocols

(Rick Simeone) #1

  1. Chapel A, Kieffer-Jaquinod S, Sagne C,
    Verdon Q, Ivaldi C, Mellal M, Thirion J,
    Jadot M, Bruley C, Garin J, Gasnier B, Journet
    A (2013) An extended proteome map of the
    lysosomal membrane reveals novel potential
    transporters. Mol Cell Proteomics 12
    (6):1572–1588. https://doi.org/10.1074/
    mcp.M112.021980

  2. Nada S, Hondo A, Kasai A, Koike M, Saito K,
    Uchiyama Y, Okada M (2009) The novel lipid
    raft adaptor p18 controls endosome dynamics
    by anchoring the MEK-ERK pathway to late
    endosomes. EMBO J 28(5):477–489.https://
    doi.org/10.1038/emboj.2008.308

  3. Sancak Y, Bar-Peled L, Zoncu R, Markhard AL,
    Nada S, Sabatini DM (2010) Ragulator-Rag
    complex targets mTORC1 to the lysosomal
    surface and is necessary for its activation by
    amino acids. Cell 141(2):290–303.https://
    doi.org/10.1016/j.cell.2010.02.024

  4. Bar-Peled L, Schweitzer LD, Zoncu R, Sabatini
    DM (2012) Ragulator is a GEF for the rag
    GTPases that signal amino acid levels to
    mTORC1. Cell 150(6):1196–1208.https://
    doi.org/10.1016/j.cell.2012.07.032

  5. Kawabuchi M, Satomi Y, Takao T,
    Shimonishi Y, Nada S, Nagai K,
    Tarakhovsky A, Okada M (2000) Transmem-
    brane phosphoprotein Cbp regulates the activ-
    ities of Src-family tyrosine kinases. Nature 404
    (6781):999–1003.https://doi.org/10.1038/
    35010121

  6. Steinberg BE, Huynh KK, Brodovitch A,
    Jabs S, Stauber T, Jentsch TJ, Grinstein S
    (2010) A cation counterflux supports lyso-
    somal acidification. J Cell Biol 189
    (7):1171–1186. https://doi.org/10.1083/
    jcb.200911083

  7. Zoncu R, Bar-Peled L, Efeyan A, Wang S,
    Sancak Y, Sabatini DM (2011) mTORC1
    senses lysosomal amino acids through an
    inside-out mechanism that requires the vacuo-
    lar H(þ)-ATPase. Science 334
    (6056):678–683. https://doi.org/10.1126/
    science.1207056

  8. Kahn BB, Alquier T, Carling D, Hardie DG
    (2005) AMP-activated protein kinase: ancient
    energy gauge provides clues to modern under-
    standing of metabolism. Cell Metab 1
    (1):15–25. https://doi.org/10.1016/j.cmet.
    2004.12.003

  9. Steinberg GR, Kemp BE (2009) AMPK in
    health and disease. Physiol Rev 89


(3):1025–1078. https://doi.org/10.1152/
physrev.00011.2008


  1. Carling D, Mayer FV, Sanders MJ, Gamblin SJ
    (2011) AMP-activated protein kinase: nature’s
    energy sensor. Nat Chem Biol 7(8):512–518.
    https://doi.org/10.1038/nchembio.610

  2. Zhao J, Hu C, Zeng J, Zhao Y, Zhang J,
    Chang Y, Li L, Zhao C, Lu X, Xu G (2014)
    Study of polar metabolites in tobacco from
    different geographical origins by using capillary
    electrophoresis–mass spectrometry. Metabolo-
    mics 10(5):805–815. https://doi.org/10.
    1007/s11306-014-0631-4

  3. Zhao Y, Zhao J, Zhao C, Zhou H, Li Y,
    Zhang J, Li L, Hu C, Li W, Peng X, Lu X,
    Lin F, Xu G (2015) A metabolomics study
    delineating geographical location-associated
    primary metabolic changes in the leaves of
    growing tobacco plants by GC-MS and
    CE-MS. Sci Rep 5:16346.https://doi.org/
    10.1038/srep16346

  4. Rui Y, Xu Z, Lin S, Li Q, Rui H, Luo W, Zhou
    HM, Cheung PY, Wu Z, Ye Z, Li P, Han J, Lin
    SC (2004) Axin stimulates p53 functions by
    activation of HIPK2 kinase through multi-
    meric complex formation. EMBO J 23
    (23):4583–4594. https://doi.org/10.1038/
    sj.emboj.7600475

  5. Rousset CI, Leiper FC, Kichev A, Gressens P,
    Carling D, Hagberg H, Thornton C (2015) A
    dual role for AMP-activated protein kinase
    (AMPK) during neonatal hypoxic-ischaemic
    brain injury in mice. J Neurochem 133
    (2):242–252. https://doi.org/10.1111/jnc.
    13034

  6. McCullough LD, Tarabishy S, Liu L,
    Benashski S, Xu Y, Ribar T, Means A, Li J
    (2013) Inhibition of calcium/calmodulin-
    dependent protein kinase kinase beta and
    calcium/calmodulin-dependent protein kinase
    IV is detrimental in cerebral ischemia. Stroke
    44(9):2559–2566.https://doi.org/10.1161/
    STROKEAHA.113.001030

  7. Faupel RP, Seitz HJ, Tarnowski W,
    Thiemann V, Weiss C (1972) The problem of
    tissue sampling from experimental animals with
    respect to freezing technique, anoxia, stress
    and narcosis. A new method for sampling rat
    liver tissue and the physiological values of gly-
    colytic intermediates and related compounds.
    Arch Biochem Biophys 148(2):509–522


Analysis of Lysosomal AMPK Activation 411
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