- Keep the kidney slices in the containers either overnight at 4C
or at room temperature for 4 h. - To proceed with the kidney slice tissue processing at the end of
the fixation time period, remove fixative from the vial (dispose
of fixative in the hood according to standard operating proce-
dures in the laboratory) (seeNote 15). - Place tissue in 1PBS for 15 min at RT and repeat twice.
Fig. 3Kidney slice preparation of the harvested kidney. (a) Place the 5 mm section of kidney on the Stadie-
Riggs slicer. (b) Cap the slicer. (c) Tighten knobs until kidney section is held firmly in place. (d) Insert the blade
into the Stadie-Riggs slicer. (e,f) Cut horizontally through the kidney slice, slicing into 0.5 mm section, in a
right-to-left motion. (g) Remove the top of the Stadie-Riggs slicer. (h) Add slice of kidney to equilibrated Ringer
solution to incubate before treatment
458 Renee Rao et al.