the control and treated tissue will likely show the same degree
of AMPK activation.- Depending on whether the protein of interest is intracellular or
in the plasma membrane, different centrifugation techniques
can be used [19].
Acknowledgments
This work was in part supported by the Wright Foundation, the
Dean’s Pilot Project Program, and the Department of Medicine at
the Keck School of Medicine of USC. We thank Dr. Kennneth
R. Hallows, Dr. Alicia McDonough, and Dona Ralph for technical
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AMPK Studies using Kidney Slices 463